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Immunohistochemistry at E14.5 for PAX6 (A,B, quantified in C) , pHH3 (D-F) , and <t>TBR2</t> (G-I) . Cortical layers markers CTIP2 (J-L) and CUX1 (M-O) are shown at E18.5 along with EdU-positive cells labeled at E13.5 (P-R). Scale bars in A,B,D,E,G,H = 50um, J,K,M,N,P,Q = 100 um.
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Immunohistochemistry at E14.5 for PAX6 (A,B, quantified in C) , pHH3 (D-F) , and <t>TBR2</t> (G-I) . Cortical layers markers CTIP2 (J-L) and CUX1 (M-O) are shown at E18.5 along with EdU-positive cells labeled at E13.5 (P-R). Scale bars in A,B,D,E,G,H = 50um, J,K,M,N,P,Q = 100 um.
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Immunohistochemistry at E14.5 for PAX6 (A,B, quantified in C) , pHH3 (D-F) , and TBR2 (G-I) . Cortical layers markers CTIP2 (J-L) and CUX1 (M-O) are shown at E18.5 along with EdU-positive cells labeled at E13.5 (P-R). Scale bars in A,B,D,E,G,H = 50um, J,K,M,N,P,Q = 100 um.

Journal: medRxiv

Article Title: NUBP2 deficiency disrupts the centrosome-check point in the brain and causes primary microcephaly

doi: 10.1101/2025.01.16.25320041

Figure Lengend Snippet: Immunohistochemistry at E14.5 for PAX6 (A,B, quantified in C) , pHH3 (D-F) , and TBR2 (G-I) . Cortical layers markers CTIP2 (J-L) and CUX1 (M-O) are shown at E18.5 along with EdU-positive cells labeled at E13.5 (P-R). Scale bars in A,B,D,E,G,H = 50um, J,K,M,N,P,Q = 100 um.

Article Snippet: Cells positive for CC3, PHH3, PAX6, and TBR2 were counted in the NIS-Elements Analysis program (Nikon, Melville NY) by manually drawing a Region of Interest (ROI) across the cortex from the ventricular zone to the pial surface and counting immune-positive cells using a bright spot detection function.

Techniques: Immunohistochemistry, Labeling